Plasmid_Backbone

Part:BBa_K1945011:Design

Designed by: Sharon Lian   Group: iGEM16_CSU_Fort_Collins   (2016-10-21)


pSB1C3 HR Plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 726
    Illegal suffix found at 754
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 726
    Illegal SpeI site found at 755
    Illegal PstI site found at 769
    Illegal NotI site found at 732
    Illegal NotI site found at 762
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 726
    Illegal XhoI site found at 2512
    Illegal XhoI site found at 3404
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 726
    Illegal suffix found at 755
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 726
    Plasmid lacks a suffix.
    Illegal XbaI site found at 741
    Illegal SpeI site found at 755
    Illegal PstI site found at 769
    Illegal NgoMIV site found at 1237
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1352


Design Notes

Source

References